Preparative Thin-Layer (Planar) Chromatography

The various OPLC andRPC techniques are compared in Table 1. Study of the data shows that OPLC is an excellent technique for analytical separations and that RPC is more ideally suited as a preparative method for isolation of compounds from biological matrices. The advantage of combining online and ofSine separations and two-dimensional development can also be exploited in OPLC. The advantage of multiple development methods is the possibility of analytical RPC separations. A realistic means of increasing the efRciency of the planar chromatography of complex samples is the use of long-distance OPLC for analytical separations and sequential RPC for preparative purposes.Workingwith multi-layer OPLC, the rapidity of the separation can increase signiRcantly, providing new vistas in screening and genetic work. FFPC techniques will open up a new Reld of planar chromatography, particularly in the separation of complex samples. It is expected that future research will concentrate on the positive effects (applied pressure in OPLC and higher centrifugal force in RPC) of forced Sow. As a consequence, smaller particle size, narrower distribution range, and spherical stationary phases will be needed to achieve maximum resolution.